Vitrification of pig embryos for the worldwide dissemination of genetics using embryo technology

Offentlig prosjektsammendrag

As an international pig breeding company, Norsvin is dependent on exporting genetics worldwide. Currently, this is mainly achieved with export of semen doses, but it slows down the customers' progress by at least 50% compared to export of live animals. However, distribution of genetics through live animals has several challenges, so as a global breeding company we see distribution through embryos as the future solution. We will then obtain a faster increase in genetic gain, higher biosecurity and minimized risk of disease transmission, increased animal welfare and reduced carbon footprint, which all together positively impacts sustainable food production. However, implementation of embryo transfer in pig breeding is still a challenge, among other things due to the large number of embryos required for a successful embryo transfer and synchronization of the recipient sows. The ability to vitrify embryos will solve those challenges and will make it possible for us to distribute genetics through embryos. Therefore, the objective of this project is to establish and optimize pig embryo vitrification protocols that eventually can be used on farm. Within this project we will first gain knowledge about vitrification of embryos to obtain the first live born piglets after non-surgical transfer in Norway. Furthermore, we will optimize freezing and thawing curves by using mathematical modelling for achieving the highest possible embryo survival so that the protocols can be used on farm. In vitro embryo quality parameters will be assessed from the most optimal protocols, followed by an in vivo non-surgical embryo transfer study with vitrified embryos. If results are not promising, changes will be made again with the mathematical modelling. This project will provide us with new expertise, and it will contribute to the final implementation of embryo technology in pig breeding. This will greatly increase the competition advantages of Norsvin as a global pig breeding company.