GenomeStrands: High efficiency synthesis of long linear single-stranded DNA HDR templates

Offentlig prosjektsammendrag

Single-stranded DNA is the preferred substrate for precise gene repair following CRISPR/Cas9 cleavage. An important factor in precise gene repair is template length, longer single-stranded DNA fragments are more efficiently incorporated into the genome. Using present technologies, longer single-stranded DNA fragments are difficult and prohibitively expensive to synthesize. GenomeStrands is our attempt to develop a new process for producing single-stranded DNA substrates. GenomeStrands will be longer, made at a higher-yield, and a fraction of the cost of present solutions. GenomeStrands will allow customers to conduct more experiments at higher efficiencies, while reducing customers' cost. Due to the involvement of CRISPR/Cas9, demand for these types of DNA substrates is large; however, the cost of competing solutions is excessive, providing a large market opportunity for a cheaper and better solution.